Ripoll says that the advance consists of being able to follow the development of these organisms, which normally appear opaque when viewed with a conventional microscope because they diffuse a lot of light when they approach adulthood. “It helps us visualize new stages,” says Ripoll. In this he way, he says, although “this technique cannot be used on living humans because our tissue is very opaque, it can be used to “take three-dimensional measurements of biopsies, which is very valuable to a surgeon,” as it would permit her to know if the surgery went as desired.
The way to put this technique into practice is simple, says Ripoll. “It consists of a source of light that stimulates the fluorescence and a camera that detects it” and has only one requirement: “that the sample rotates” as if X-rays were being taken of it. Afterwards, with that information, “we must construct a three-dimensional image,” he explains.
The development of this technique has been possible thanks to the support, among others, of researchers from the Chinese Academy of Sciences. These researchers “were responsible for creating the software so that the obtaining of images could be fast and effective,” he says. Along with this, he comments that the technology on which the techniques his Chinese colleagues use are based has its origins in the development of video games.
Participating in this research are Alicia Arranz, from the Swiss Federal Institute of Technology; Di Dong, from the Chinese Academy of Sciences; Shouping Zhu and Jie Tian, from the School of Life Sciences and Technology; Charalambos Savakis, from the BSRC Alexander Fleming and Jorge Ripoll, from the UC3M Department of Bioengineering and Aerospace Engineering.